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To achieve the purpose of this study, forty-eight male Sprague-Dawley rats were assigned to control and three endurance exercise group. 36 rats were forced to exercise according to exercise intensity for 8 weeks and 12 rats were untrained for control group. Cardiac cytosol was extracted from cardiac tissue and cardiac mitochondria was purified from the cytosol. Purified mitochondria were separated into four fraction: inner membrane, outer membrane inter membrane space and matrix. The changes of cytosolic and mitochondrial LDH isozymes activity were measure. Relative activity (%) of cytosol for low and control group showed the following order of prevalence : AB₃＞A₂B₂＞B₄＞A₃B＞A₄, for moderate and high group : AB₃＞B₄＞A₂B₂＞A₃B＞A₄. Outer membrane for low group showed AB₃＞B₄＞A₂B₂, for moderate group : B₄＞AB₃＞A₂B₂, for high and control group : B₄＞A₃B. Inter membrane space for low, moderate and high group showed B₄＞AB₃＞A₂B₂＞A₃B＞A₄, for control group : B₄＞A₃B＞AB₃＞A₂B₂＞A₄. Inner membrane for all group showed B₄＞AB₃＞A₂B₂＞A₃B＞A₄. Matrix for control, low, moderate and high group showed B₄＞AB₃＞A₂B₂＞A₃B＞A₄. These results suggest that long term exercise intensity effect on cardiac tissue cytosolic and mitochondrial activity and A₄, A₃B, A₂B₂, AB₃and B₄isozymes were found entirely in mitochondrial fraction.
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